Homeodomain proteins in action: similar DNA binding preferences, highly variable connectivity
نویسندگان
چکیده
منابع مشابه
Differential DNA binding properties of three human homeodomain proteins.
The products of three human homeobox containing (HOX) genes, 2C, 3C and 4B, were produced in insect cells using the Baculovirus expression system and purified to near homogeneity. In this system we observed that the DNA binding forms of the three proteins are not glycosylated. HOX 3C and 4B are phosphorylated in insect cells, while HOX 2C is not. The three HOX proteins bind to a DNA sequence kn...
متن کاملRecognition models to predict DNA-binding specificities of homeodomain proteins
MOTIVATION Recognition models for protein-DNA interactions, which allow the prediction of specificity for a DNA-binding domain based only on its sequence or the alteration of specificity through rational design, have long been a goal of computational biology. There has been some progress in constructing useful models, especially for C(2)H(2) zinc finger proteins, but it remains a challenging pr...
متن کاملVariation in Homeodomain DNA Binding Revealed by High-Resolution Analysis of Sequence Preferences
Most homeodomains are unique within a genome, yet many are highly conserved across vast evolutionary distances, implying strong selection on their precise DNA-binding specificities. We determined the binding preferences of the majority (168) of mouse homeodomains to all possible 8-base sequences, revealing rich and complex patterns of sequence specificity and showing that there are at least 65 ...
متن کاملDiversity of graphs with highly variable connectivity.
A popular approach for describing the structure of many complex networks focuses on graph theoretic properties that characterize their large-scale connectivity. While it is generally recognized that such descriptions based on aggregate statistics do not uniquely characterize a particular graph and also that many such statistical features are interdependent, the relationship between competing de...
متن کاملDNA binding specificity of two homeodomain proteins in vitro and in Drosophila embryos.
In previous experiments, the homeodomain proteins even-skipped and fushi-tarazu were found to UV cross-link to a surprisingly wide array of DNA sites in living Drosophila embryos. We now show that UV cross-linking gives a highly accurate measure of DNA binding by these proteins. In addition, the binding of even-skipped and fushi-tarazu proteins has been measured in vitro to the same DNA fragmen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Opinion in Genetics & Development
سال: 2017
ISSN: 0959-437X
DOI: 10.1016/j.gde.2016.09.008